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Table 2: Estimates of historical demographic parameters in various green toad taxa and groups from mtDNA control region analyses using parameter estimation in the programs
Fluctuate and DnaSP.

Mitochondrial Taxon N Theta* g @ Ln (like-lihood) ln (likely-hood) 2(L max - L = 0) No growth can MinAge estimate for MinAge estimate Tajima's D #
g
control region clade for L max for zero growth be rejected corrected rate (pi-Net) for non-corrected
rate (pi-betw.)

[All B. boulengeri: B. boulengeri 37 0.0258 175.878 0.0041 -2.0458 4.0998 Yes 1.6 My 0.9 My -0.66664
North Africa
including islands]
North Africa B. boulengeri 18 0.0377 263.037 0.1060 -3.5830 7.378 Yes 2.2 My 1.4 My -1.63869
mainland

Sicily and adjacent B. siculus n. sp. 33 0. 0015 2585.02 0.6440 0.7064 -0.1248 No NA NA 0.74861
islands
[All B. balearicus: B. balearicus. 61 0.0116 184.754 0.5139 0.1018 0.8242 No NA NA 0.74773
Apennine Peninsula,
Corsica, Sardinia,
Balearic islands]

Corsica, Sardinia B. balearicus. 21 0.0177 51.788 0.0455 -0.1783 -0.447 No NA NA -0.07834
Apennine Peninsula B. balearicus. 29 0.0029 767.136 0.0364 -0.2254 0.5236 No NA NA 0.19720
BMC Evolutionary Biology 2008, 8:56 # all values non-significant
* = 2N µ estimate of the distribution of coalescence times in the population at the time the sample was taken, estimated by Fluctuate
e
@ = exponential growth parameter estimated by Fluctuate.