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TABLE 2. Details of the COI and 16S reference sequences of Kyphosus species downloaded and showed in the NJ tree.
Accession Number (A.N.) Sampling site Reference
and Specimen Voucher
COI
Kyphosus vaigiensis
JQ431874 (voucher MBIO207.4) South Pacific Ocean (Moorea, French Polynesia) Hubert et al. (2012)
Zhang & Hanner (2011)
JF952770 (voucher ISZ9) North Pacific Ocean (Yokohama, Japan) sequence unpublished (1)
Valdez-Moreno et al. (2010)
JF493714 (voucher Smith 189.3-2) South Indian Ocean (KwaZulu-Natal, South Africa)
Weigt et al. (2012)
GU224526 (voucher MFL1494) North Atlantic Ocean (Gulf of Mexico, Belize) Deeds et al. (2014)
sequence unpublished (2)
Kyphosus incisor [syn. vaigiensis sensu Knudsen & Clements (2013a)] Ribeiro et al. (2012)
JQ841613 North Atlantic Ocean (Gulf of Mexico, Belize)
KF461190 North Atlantic Ocean, (Gulf of Mexico, USA)
JX124794 South Atlantic Ocean (Brazil)
JQ365389 South Atlantic Ocean (Brazil)
Kyphosus sectatrix
JQ365390 South Atlantic Ocean (Brazil) Ribeiro et al. (2012)
JQ839801 North Atlantic Ocean (Gulf of Mexico, Belize) Weigt et al. (2012)
Kyphosus cinerascens
AP011061 North Pacific Ocean (Japan) Yagishita et al. (2009)
Kyphosus bigibbus South Indian Ocean (Walters Shoal, South Africa) sequence unpublished (3)
KF489620
16S
Kyphosus vaigiensis
AY530871 South Pacific Ocean (Great Barrier Reef, Australia) Bellwood et al. (2004)
KC136382 Mediterranean Sea, eastern Ligurian Sea (Italy) Knudsen & Clements (2013a)(4)
Kyphosus cinerascens
AP011061 North Pacific Ocean (Japan) Yagishita et al. (2009)
Kyphosus sectatrix
JN007799 Mediterranean Sea, Ionian Sea (Greece) Kiparissis et al. (2012)
Kyphosus bigibbus
KC136508 South Pacific Ocean (Western Australia) Knudsen & Clements (2013b)
(1) uploaded by Steinke, D., Zemlak, T.S., Connell, A.D., Heemstra, P.C. & Hebert, P.D.N.—University of Guelph, Canada.
(2) uploaded by Ribeiro, A.O. & Oliveira, C.—Universidade Estadual Paulista, Brazil.
(3) uploaded by Steinke, D., Connell, A.D. & Zemlak, T.S.—University of Guelph, Canada.
(4) specimen recorded by Ligas et al. (2011) and erroneously identified as K. sectatrix (Knudsen & Clements, 2013a).
Sequence alignment and successive analyses were implemented with MEGA 6 software (Tamura et al., 2013).
Measuring of genetic differentiation was based on Kimura-two-Parameter (K2P) model (Kimura, 1980). Unrooted
Neighbor-Joining (NJ; Saitou & Nei, 1987) trees were built and the nodes were supported by a high proportion (>
90%) of replicates in the bootstrap analysis (Felsenstein, 1985).
The Automatic Barcode Gap Discovery (ABGD) analysis (Puillandre et al., 2012) was used to detect a barcode
gap in the COI dataset. The COI sequences were uploaded to the ABGD web interface and were run with the
default settings. K2P distance was tested to infer from the data a model-based confidence limit for maximum
48 · Zootaxa 3963 (1) © 2015 Magnolia Press MANNINO ET AL.
