Page 107 - Dedola2011
P. 107
3. Results
3.1. COI results
Based on a total of 197 sequences (65 ofwhich were obtained in this research) 437 bp
long, ML retrieved three main clusters (Figure 3): A) a basai clade which groups
together P. vulgata specimens; B) a clade grouping all P. ulyssiponensis s. l. specimens,
which further splits in two distinct clusters, "continental" and "Macaronesian" P.
ulyssiponensis (Bl and B2, respectively); and C) a monophyletic clade which
encompasses P. caerulea and P. candei specimens, with the former species (Cl) nested
within two distinct clusters of the second that include P. candei from Canaries (C2) and
P. candei from Azores (C3). BI was consistent with ML in showing the same tree
topology, with the exception ofthe cluster which grouped P. ulyssiponensis s.I., in
which individuals of the "continental" form did not form a monophyletic clade
supported by an high posterior probability value (Figure 3).
Sixty-five Patella specimens sampled were thus identified (Figure 3): 33 individuals
belonged to P. ulyssiponensis s.I., 16 to P. caerulea, six to P. candei, and ten to P.
vulgata (see Table II for details). In particular, our P. ulyssiponensis s.l. specimens
grouped into the cluster B l or B2 of the ML tree (Figure 3), depending on their
"continental" or "Macaronesian" provenience. As well, our P. candei specimens from
Tenerife clustered within the C2 branch.
The K2P average distances showed the existence of barcoding gaps, since interspecific
distances, comprised between 36.079 (P. caerulea vs. P. candei) and 82.521 (P. vulgata
vs. P. candei) were much higher than the intraspecific ones, comprised between 1.350
(P. vulgata) and 18.358 (P. candei) (Table III).
3.2. RFLP results
107
Gìan Luca Dedola: ANALISI DELLA VARIABILITÀ GENETICA DI PATELLA FéRRUGINEA, PATEL-LA UL l'SSIPONENSIS (MOLLUSCA: GASTROPODA) E PINNA
NOBILIS (MOLLUSCA: BIVALVIA): lLCONTRJBUTO DEl DATI MOLECOLARl ALLA CONSERVAZIONE DI SPECIE MINACCIATE · Tesi di douorato in Scienze
della natura e delle sue risorse • indiri7.zo BK>logi.a Ambientale, Università degli Studi di Sassarl
