Page 60 - Dedola2011

Tbe mismatcb analysis carried out on the whole dataset (Fig. 6a) showed an
incipient bell-shape curve. A similar trend is shown by analysis of SES (Fig. 6b) and
VL (Fig. 6c) groups while a starting shape curve occurs in the mismatch distribution of
GT group (Fig. 6d).

Outcomes provided by mismatch analysis were confirmed by the statistica]
significance - with the only exception ofVenetian population as probably consequence
of its reduced size- ofTajima's D and Fu's FS negative values ofthe neutrality tests
(Table 5).

16S
A total of 199 individuals were analysed obtaining a 497 bp long sequences

alignrnent. With the only exception ofNetwork analysis, such dataset has been analysed
without comparison with other Mediterranean populations since the only sequences of
this gene available at the moment for Pinna nobilis correspond to the two haplotypes-
whose sequences are eighth base pairs shorter than ours (490 bp) - found by Katsares et
al. (2008) among Grecian populations.

JModelTest calculated as appropriate model of nucleotide substitution the
custom model TrN+G with gamma shape y = 0.0110.

Tbe 29 polymorphic sites (S) found defined 34 different haplotypes with an
average haplotype diversity (h) equal to 0.542 and a correspondent average nucleotide
diversity (n) equal to 0.002 (Table 6).

Median-Joining network analysis (Fig. 7) was carried out including in our
dataset 25 sequences belonging to Grecian populations (Katsares et al. 2008) and
representing the only two 16S haplotypes known till now for this species. The most
diffused haplotypes (G l) characterises populations of Epanomi, Aggeloxori and

60

Gìan Luca Dedola: ANALISI DELLA VARIABILITÀ GENETICA DI PATELLA FéRRUGINEA, PATEL-LA UL l'SSIPONENSIS (MOLLUSCA: GASTROPODA) E PINNA
NOBILIS (MOLLUSCA: BIVALVIA): lLCONTRJBUTO DEl DATI MOLECOLARI ALLA CONSERVAZIONE DI SPECIE MINACCIATE - Tesi di douorato in Scienze
della natura e delle sue risorse · indiri7.zo BK>logi.a Ambientale, Università degli Studi di Sassarl