Page 61 - Dedola2011
P. 61

Korinthiakos, while the less diffused one (G2) characterises populations of Xios. In
order to analyse sequences of equallength, we excluded from the analysis the first eight
bp fragments from our sequence dataset obtaining a fina! 490 bp long sequences
alignment. Network graphic output provided a star-like configuration with a main
haplotype shared by 49% of individuals differing from surrounding haplotypes,
including Grecian ones, for one/two point mutations only. No evidence of geographic
structuring occurred neither at !oca! scale considering singularly ali populations (data
not shown) nor at larger scale grouping populations in seven macro-areas (SAR, SIC,
ELBV, VL, CYP, BIZ and GRE), with the only exception of the Grecian populations
which do not share haplotypes with others. It is interesting to note that a Cyprian
haplotype appears included in the Grecian variability differing for a single point
mutation from Grecian founder haplotype.

         The Bayesian analysis evidenced the occurrence of three distinct groups of
haplotypes (Fig. 8). The first group, shared by 69% of individuals, is present in almost
all populations analysed. The second group, shared by 16% of individuals, is less
diffused being totally absent in four Sardinian populations (SAL, MP, MAR NAS
VSM), one Sicilian population (SVC) and in the Venetian Lagoon. The third group,
shared by 15% of individuals, is almost exclusively of Venetian Lagoon being also
present in six Sardinian populations which are mostly located in the North-eastem
coastline (MO, SAL, CC, OTT, IMV AND VSM).

         The mismatch analysis carried out on the whole dataset (Fig. 9) showed a
starting shape curve. The same trend characterises all the populations analysed (data not
shown).

                                                                                                           61

Gìan Luca Dedola: ANALISI DELLA VARIABILITÀ GENETICA DI PATELLA FéRRUGINEA, PATEL-LA ULl'SSIPONENSIS (MOLLUSCA: GASTROPODA) E PINNA
NOBILIS (MOLLUSCA: BIVALVIA): lLCONTRJBUTO DEl DATI MOLECOLARl ALLA CONSERVAZIONE DI SPECIE MINACCIATE - Tesi di douorato in Scienze
della natura e delle sue risorse • indiri7.zo BK>logi.a Ambientale, Università degli Studi di Sassarl
   56   57   58   59   60   61   62   63   64   65   66