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BMC Evolutionary Biology 2008, 8:56                            http://www.biomedcentral.com/1471-2148/8/56




            Background                                          in: [22]]. However,  Bonfiglio [14] also hypothesized a
            On the phylogeographic patterns of terrestrial Sicilian   Lower Pleistocene African origin of fossil elephants
            fauna                                               (Elephas falconeri) as a debatable alternative to derivation
            Since  Busack [1,2], the faunal  relationships  between  from European/Italian  E. antiquus  [Bonfiglio in: [22]].
            North Africa and Iberia have been the focus of numerous  Other paleontologists have also discussed whether Sicily
            molecular phylogeographic studies, yet little attention has  had early Pleistocene connections to Africa, as in an, in
            been drawn to the consequences that the last landbridge  this respect, unresolved investigation of  a  Pleistocene
            between Africa and Sicily may have had on Mediterranean  amphisbaenid  lizard from Sicily [23]. All  these  authors
            biogeography. As was the Strait of Gibraltar, the Strait of  [22-24], however, refer to the lack of conclusive evidence
            Sicily (no universally accepted  name  in English;  other  for a Pleistocene landbridge. Recent molecular data have
            names include Sicilian Strait, Sicilian Channel, Channel  suggested phylogeographic links across the Strait of Sicily,
            of  Sicily,  Pantelleria Channel) is  purported to have  either based on very little data, or, with some speculation
            formed  at the  end of the  Messinian salinity crisis (5.3  on the dating of these divergences [[20,25]; see Discus-
            Mya), at the Miocene/Pliocene boundary  [e.g. [3-6], F.  sion].
            Rögl pers. comm.]. The Messinian [4] was a geological
            period from 5.59 to 5.33 Mya during which the Mediter-  On green toads in the western Mediterranean region and
            ranean Sea was isolated from the Atlantic Ocean, resulting  North Africa
            in a large decrease in the Mediterranean Sea level and the  Green toads are widespread in the Palearctic region where
            formation  of landbridges between Africa, Europe and  they have differentiated into  several lineages [26]. The
            most Mediterranean islands. This included a well-docu-  occurrence of three bisexually reproducing ploidy levels
            mented landbridge between Africa and a landmass that  [27] makes them a uniquely interesting vertebrate group.
            later became part of Sicily, which may be the last terres-  A recent survey of mtDNA variation characterized a deeply
            trial connection  between the African mainland and the  branched assemblage of at least twelve major haplotype
            island. However, while it is known that Sicily and Tunisia  groups [28]. Other data on green toad variation from
            are at present approximately  140 km apart, low Pleis-  North Africa have been relatively scarce [29,30]. Research
            tocene sea levels of about -120 m [7-9] would have repeat-  has been mainly restricted to faunistics [31-33] or analy-
            edly drawn the north African paleo-coast and the Sicilian  ses of single populations (Egypt [34]) without taxonomic
            landmass closer than ~50 km (Figure 1). In addition, cur-  or phylogeographic focus. Green toad biology and ecol-
            rent shoals [10,11] may be remnants of Pleistocene "step-  ogy are relatively well known from the Balearic Islands
            ping stone islands" that may have facilitated terrestrial  [35,36], Corsica [[37] incl. refs.], Sardinia and mainland
            animals, including humans [12], in overcoming the sea  Italy [38]. In addition, morphometric analyses of green
            barrier. Therefore, the phylogenetic depths of sister rela-  toads in Italy have been restricted to peninsular and Sar-
            tionships between Sicily and Africa may vary as they do  dinian (plus Corsican) populations [38,39] until mor-
            for trans-Gibraltarian relationships [2,13]. Observations  phometric data for a population from northwestern Sicily
            of this pattern in widespread taxa might be explained by  became available [40]. Otherwise, regional research has
            multiple invasions across the Strait of Sicily and/or addi-  been restricted to classical biogeography and faunistics
            tional potential invasions from the region that now forms  including ecological and taxonomic remarks [41-43] and
            the Italian (Apennine) Peninsula.                   studies on phenology [40,44].

            As expected for an island with a long Pliocene isolation  To gain insight into the phylogeography of the region, we
            [14], endemic (island specific) forms in several animal  test a wide range of possible substitution rates in order to
            groups on Sicily have been revealed by molecular analyses  better date the African-Sicilian divergence of green toads:
            (e.g.: hedgehogs,  Erinaceus  [15]; shrews, Crocidura  [16];  Based on only two haplotypes from Sicily, Stöck et al. [28]
            mice, Apodemus [17]; beetles, Pachydemidae [18]; terra-  roughly  calibrated their tree by "assuming  that  the  last
            pins, Emys [19]; skinks, Chalcides [20]). Using these meth-  landbridge between North Africa and Sicily broke about
            ods, some of these species have been shown to be sister  5.3 Mya." Alternatively, this divergence could be younger.
            taxa to populations  from the Apennine Peninsula  (e.g.  Here, we examine genetic data from many individuals and
            Apodemus, Erinaceus) or  to  be of very  recent Apennine  many more localities in Tunisia, Italy and Sicily, to scruti-
            peninsular (or Calabrian) origin (reviewed in [21]). This  nize the North Africa-Sicily divergence employing a Baye-
            pattern may be explained by "an intermittent filter barrier  sian coalescent  demographic reconstruction method
            in the area of the Strait of Messina [which] probably con-  (BEAST 1.4.6). Using two mitochondrial and two nuclear
            trolled the processes and timing of the Late Middle Pleis-  sequence markers and additional morphological, pheno-
            tocene-Late Glacial vertebrate faunal dispersal in Sicily"  logical, bioacoustic and biogeographic data, we character-
            [Bonfiglio in: [22]]. Many Pleistocene fossil megafaunal  ize green toads from Sicily as a  separate evolutionary
            elements entered Sicily from peninsular Italy [Bonfiglio  lineage that is a sister taxon to African green toads, but


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