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           Ann Microbiol (2015) 65:1–13                                                                      5

                  a
                                                             1.8

                                                          Faraglioni-shore-line
                                                             1.5
                                                           Faraglioni-shore-line
                                                             1.2
                                                         Faraglioni-shore-line
                                                             0.9    Organic Carbon
                                                                                    Faraglioni-mid-line
                   Axis 2                                    0.6      Faraglioni-mid-line  Faraglioni-mid-line
                                                                            Faraglioni-upper-line
                                               Humidity      0.3                  Faraglioni-upper-line
                                                                       Faraglioni-upper-line
                      -2.0    -1.6    -1.2    -0.8    -0.4            0.4     0.8     1.2     1.6
                           Lido_Burrone-shore-line  Praja-shore-line  Praja-shore-line  Lido_Burrone-upper-line
                                                             -0.3  Lido_Burrone-upper-line
                                                                     Lido_Burrone-upper-line
                                                          Praja-shore-line
                                           Praja-mid-line               Lido_Burrone-mid-line
                       Lido_Burrone-shore-line         Praja-mid-line  Lido_Burrone-mid-line
                                                             -0.6
                                      Lido_Burrone-shore-line      Lido_Burrone-mid-line  Praja-upper-line
                                                               Praja-mid-line
                                                                                          Praja-upper-line
                                                             -0.9                Praja-upper-line
                                                              Axis 1
                  b
                                               Jaccard similarity  0.7  0.8  0.9

                             0.2
                                                                              1
                                   0.3
                                                      0.6
                                                0.5
                                         0.4
                                                                                Praja-shore-line
                                                                                Praja-shore-line
                                                                                Praja-shore-line
                                                                                Praja-mid-line
                                                                                Lido_Burrone-shore
                                                                                Praja-mid-line
                                                                                Praja-mid-line
                                                                                Lido_Burrone-shore
                                                                                Lido_Burrone-shore
                                                                                Praja-upper-line
                                                                                Praja-upper-line
                                                                                Praja-upper-line
                                                                                Lido_Burrone-mid-line
                                                                                Lido_Burrone-mid-line
                                                                                Lido_Burrone-mid-line
                                                                                Faraglioni-shore-line
                                                                                Faraglioni-shore-line
                                                                                Faraglioni-shore-line
                                                                                Faraglioni-mid-line
                                                                                Faraglioni-mid-line
                                                                                Faraglioni-mid-line
                                                                                Lido_Burrone-upper-line
                                                                                Lido_Burrone-upper-line
                                                                                Faraglioni-upper-line
                                                                                Lido_Burrone-upper-line
                                                                                Faraglioni-upper-line
                                                                                Faraglioni-upper-line
           Fig. 1 Pattern of ordination of 16S rRNA bacterial community T-RFLP  Lido Burrone; squares, mid-line samples; circles, shore-line samples;
           profiles. a Nonmetric Muldimensional Scaling (nMDS); b) UPGMA  triangles, upper-line samples. Black-filled symbols, Faraglioni, grey-filled
           clustering. Cophenetic correlation coefficient=0.9098. In CCA each  symbols, Lido Burrone; white-filled symbols, Praja. Vectors for humidity
           point represents a DNA sample for the beaches of Faraglioni, Praja and  (%) and organic carbon (%) are reported

           different localities (beaches) were detected, in particular when  dendrogram highlighted a strong differentiation of Faraglioni
           cluster analysis was applied (Fig. 1b). In fact, UPGMA  beach samples from Lido Burrone and Praja samples. In
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