Page 11 - Bacci_Pagoto_etali_2015

Author's personal copy

Ann Microbiol (2015) 65:1–13 9

Fig. 3 Identification of Acidobacteria Actinobacteria Bacteroidetes Chlamydiae
abundance trends in bacterial 800 12000 12000
Phyla along the Y-axis. Panels
600 9000 20
show the trends of abundance of 8000
main Phyla along the Y-axis. 400 6000
Shown Phyla have observed 4000 10
differences that are significant at 200 3000
P<0.05 after a resampling 0 0 0 0
analysis and Wilcoxon test. For Chloroflexi Cyanobacteria Deinococcus−Thermus Firmicutes
Number of hits 2 4 1500
each plot abscissa is indicated the 3 6
Y-axis sample, while on the 80 2000
ordinate is the number of reads 60
accounting for that phylum 1 40 2 1000

0 20 0 0 500 0
Planctomycetes Proteobacteria Spirochaetes Verrucomicrobia
60000 2
60
40000 100
40 1
20000 50
20
0 0 0 0
shore.linemid.lineupper.line shore.linemid.lineupper.line shore.linemid.lineupper.line shore.linemid.lineupper.line
Sample

5
4
(Misic and Fabiano 2005; Schlacher et al. 2008;Ugolini and number of bacterial cells (from 10 to 10 cells/g as detected
Ungherese 2012). Surprisingly, here we have shown that, despite by the qPCR approach), lower than soil but comparable to those
the low organic carbon present (for reference with agricultural found in the plant endosphere (Mengoni et al. 2005, 2009;Pini
soils, see, for instance, Ludwig et al. 2011) and the extreme et al. 2012) and lower than those found in La Jolla (California)
conditions, the sampled sandy beaches contain appreciable sandy beaches (Glavin et al. 2004) and in Mediterranean sea

Fig. 4 Cluster analysis for a FirmicutesFamilydistribution
Firmicutes and Proteobacteria. 1000
800
The analysis was performed as 600
400
those reported in the legend of 200
0
Figure 3, byinspectingthe
upper.line value
diversity at family level for 0.0
0.2
Firmicutes (a)and Proteobacteria mid.line 0.4
0.6
(b) 0.8
1.0
shore.line
Bacillaceae Paenibacillaceae Planococcaceae Clostridiaceae Lachnospiraceae Peptostreptococcaceae Ruminococcaceae
Assignments
b Proteobacteriafamilydistribution
10000
8000
6000
4000
2000
0
mid.line value
0.0
0.2
shore.line 0.4
0.6
0.8
1.0
upper.line
Methylococcaceae
Alcanivoracaceae
Ectothiorhodospiraceae
Chromatiaceae
Xanthomonadaceae
Vibrionaceae
Moraxellaceae
Caulobacteraceae Methylocystaceae Xanthobacteraceae Rhodospirillaceae Alcaligenaceae Oxalobacteraceae Desulfohalobiaceae Cystobacteraceae Syntrophobacteraceae Colwelliaceae Enterobacteriaceae Halomonadaceae Pseudomonadaceae
Oceanospirillaceae
Alteromonadaceae
Burkholderiaceae
Phyllobacteriaceae
Brucellaceae
Comamonadaceae
Sphingomonadaceae
Acetobacteraceae
Erythrobacteraceae
Rhodobiaceae
Rhodobacteraceae
Nannocystaceae
Aeromonadaceae
Polyangiaceae
Hyphomicrobiaceae
Desulfobulbaceae
Desulfobacteraceae
Desulfuromonadaceae
Desulfovibrionaceae
Assignments