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New and little known land snails from Sicily (Mollusca Gastropoda) 209
at most bifurcate with wider base, and the posterior racterized by a very long epiphallus externally si-
apex is bigger. milar to that of T. marinellii. However, T. rustica
is characterized by its whitish or creamy colora-
Examined material of Tandonia sowerbyi. Italy, tion, somewhat violetish, with numerous, small
Emilia Romagna, Castiglione dei Pepoli, Roncobi- black dots; the penial complex (epiphallus+penis)
laccio, 44°06’59’’N 11°13’42’’E, 593 m, is smaller (around 10 mm) compared to T. mari-
5.xI.2011, 2 specimens (CS). Italy, Sicily: Castel- nellii (20-31 mm), and has a different structure:
buono, Cozzo Luminario, Piano Sempria, penis proportionally shorter and epiphallus sho-
37°54’18”N 14°03’59”E, 1192 m, x.1990, 4 spe- wing internally long longitudinal rows of papillae,
cimens (CS); Palermo, Parco della Favorita, Val- penial papilla proportionally larger and much more
lone del Porco, 38°10’07”N 13°20’39”E, 243 m, ornate; the place where musculus retractor inserts
13.xI.1990, 4 specimens (CS); Monreale, Bosco is not constricted; atrium is narrow and tube-sha-
Ficuzza, Diga Scanzano, 37°55’14”N 13°22’25”E, ped while in T. marinellii is short and very broad.
536 m, 1.xII.1990, 6 specimens (CS); Palermo,
Fiume Oreto, Ponte delle Grazie, 38°04’4”N Examined material of Tandonia rustica. Italy,
13°19’25”E, 95 m, 19.xI.1990, 5 specimens (CS); Emilia Romagna, Castiglione dei Pepoli, Ronco-
idem, 3.xII.1990, 2 specimens (CS); Isnello, Con- bilaccio, 5.xI.2011, 1 specimen (CS); Italy, Lom-
trada Montaspro, 37°54’42”N 13°59’30”E, 857 m, bardia, Brescia, Valvestino, Armo, 45°46’N
5.III.1991, 1 specimen (CS); Collesano, Contrada 10°35’E, 666 m, 22.x.2000, 1 specimen (CN);
Croce, 37°55’23”N 13°55’20”E, 511 m, 5.III.1991, idem, Anfo, S. Petronilla, 45°46’N 10°29’E, 524
6 specimens (CS); Cammarata, Monte Cammarata, m, 4.V.2008, 1 specimen (CN); idem, Ghedi,
Cozzo Panepinto, 37°38’16”N 13°36’34”E, 984 m, 45°24’N 10°16’E, 85 m, 20.Ix.1996, 1 specimen
III.1992, 4 specimens (CS); idem, 2 specimens (CN), idem, Marone, Vello, 45°45’N 10°05’E, 200
(CL); Petralia Sottana, Fiume Imera Meridionale, m, 01.V.2007, 1 specimen (CN).
37°48’26”N 14°05’01”E, 808 m, 2.xI.1992, 3 spe-
cimens (CS); San fratello, Monte Soro, Pizzo MOLECULAR ANALYSIS. Seven Tandonia speci-
Muto, 37°56’16”N 14°38’16”E, 1410 m, x.2001, mens, three from Custonaci (TP) and four from
1 specimen (CS); idem, 1 specimen (CL); Melilli, Madonie mountains (PA), labelled as TCUS and
Riserva Naturale Integrale Grotta Palombara, 2008 TMAD respectively, were analyzed. Samples were
(CR); Monreale, Ponte Arcera, 37°55’42”N stored in 75% Ethanol at -20 °C in test tubes. For
13°23’01”E, 470 m, 14.xI.2008, 2 specimens, each individual, a piece of about 40-50 mg was
(CL); Melilli, Riserva Naturale Integrale Grotta Pa- used for total DNA extraction. Pieces of each spe-
lombara, 2008 (CR); Vizzini, Contrada Rubala, cimen were deposited as vouchers at University of
near the F. Vizzini, 37°08’28”N 14°44’15”E, 376 Urbino, Lab. of cytogenetics and molecular bio-
m, 11.I.2009, 5 specimens (CL); Prizzi, Fontana logy. COI amplicons (654 bp) were obtained by
Grande, 37°42’53”N 13°25’43”E, 800 m, 15.xI.2009, LCO1490/HCO2198 universal primers (5’-
4 specimens (CL); Torrenova, Rocca Scovoni, GGTCAACAAATCATAAAGATATTGG-3’/5’-
Piano Scodoni, 38°05’38”N 14°41’26”E, 25 m, TAAACTTCAGGGTGACCAAAAAATCA-3’)
06.xII.2009, 1 specimen (CL); Castelbuono, S. Gu- as in Folmer et al. (1994) with a PCR cycle of 95
glielmo, near the creek San Calogero, 37°55’04”N °C for 5 min; 95 °C for 1 min, 42 °C for 1 min, 72
14°04’22”E, 670 m, I.2010, 3 specimens (CL); No- °C for 1 min (37 cycles); 72 °C for 10 min. Se-
vara di Sicilia, Rocca Novara, 37°59’35”N quencing of the purified PCR products was carried
15°08’25”E, 1000 m, 07.xI.2010, 4 specimens out using automated DNA sequencers at Eurofins
(CL); Itala, Piano Fattaredda, 38°02’48”N MWG Operon (Germany). Finally, sequence chro-
15°25’09”E, 612 m, 12.xII.2010, 2 specimens matograms of each amplified fragment were brow-
(CL); Isnello, Vallone Montaspro, 37°54’18”N sed visually. Sequences generated in this study
13°58’55”E, 783 m, 26.II.2012, 2 specimens (CL). were analysed with additional seventy-six Lima-
cidae COI sequences retrieved from GenBank (IDs:
Tandonia rustica (Millet, 1843) has an Euro- AF239733-34, AM259702-06, AM259712-14,
pean central and southern distribution and is found EF128217, FJ481179, FJ481181, FJ606455-71,
in the northern regions of Italy and along the Apen- FJ606481, FJ606483, FJ606485, FJ606487,
nines up to the central regions. This species is cha- FJ606489, FJ606491, FJ606493, FJ606495,
