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Results population (pop7) and for B. incana populations
(pop14 and 15).
Rare alleles (frequency , 5 %) were mainly found
Allele frequencies in B. rupestris (pop7 to 10), B. villosa subsp. villosa
(pop1), B. incana (pop15) and B. macrocarpa
Eleven polymorphic loci were observed from the five (pop16). Six alleles were specifically found in one
enzymes investigated (Table 2). In particular Lap-1 population (* in Table 2).
locus showed the most variability with five different
alleles and only one population of B. villosa subsp. Genetic variability
bivoniana (pop5) being monomorphic at this locus.
The four loci belonging to the ACO system were also A total of 37 alleles was observed. For each popula-
very variable with three alleles detected for each tion the mean proportion of polymorphism levels P95
locus. Some loci were monomorphic for most popula- and P99, the mean number of alleles per locus across
tions as Pgm-3 which was polymorphic only for one all loci (A) and per polymorphic locus (Ap) and the
population of B. rupestris subsp. rupestris (pop7). Wright’s fixation index (F) are shown (Table 3).
The 6PGD system was also only polymorphic for this Mean number of alleles per locus (A) varied from

Table 2. Frequencies of the allozymes detected in 16 wild Brassica populations analyzed with 5 enzymatic systems.
System Locus Allele pop1 pop2 pop3 pop4 pop5 pop6 pop7 pop8 pop9 pop10 pop11 pop12 pop13 pop14 pop15 pop16
PGI Pgi-2 1 0.614 1.000
2 *0.386
3 *0.260
4 1.000 0.740 1.000 1.000 1.000 1.000 1.000 0.886 1.000 1.000 1.000 1.000 1.000
5 0.114 1.000
PGM Pgm-3 1 *0.071
2 1.000 1.000 1.000 1.000 1.000 1.000 0.929 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000
Pgm-1 1 *0.129
2 0.477 0.100 0.514 0.029 0.614 0.029
3 1.000 0.523 1.000 0.900 1.000 0.357 1.000 0.971 0.971 0.729 0.386 0.943 1.000 1.000 0.171 1.000
4 0.029 0.271 0.029 0.829
Pgm-2 1 0.143 0.900
2 0.128 0.628 0.100 0.271
3 0.957 0.900 0.574 0.486 1.000 1.000 1.000 0.329 1.000 1.000 0.700
4 0.043 0.100 0.426 1.000 0.514 1.000 0.929 0.543 0.229 0.029
5 *0.071
ACO Aco-1 1 0.128 0.043 0.371
2 0.857 0.900 0.279 0.414 1.000 1.000 0.957 1.000 1.000 0.928 1.000 1.000 0.629 1.000 1.000 1.000
3 0.015 0.100 0.721 0.586 0.072 0.000
Aco-2 1 0.971 0.286 0.147 0.129 0.571 0.271 0.571 0.286 0.029 0.757
2 0.029 0.714 0.853 0.871 0.429 1.000 0.729 0.429 0.514 1.000 1.000 1.000 0.614 1.000 0.971 0.243
3 0.486 0.100
Aco-3 1 0.441 0.186 0.429 0.343 0.057 0.200
19 0.914 1.000 0.559 1.000 1.000 0.686 0.357 0.471 0.529 0.657 0.643 0.500 1.000
2 0.086 0.129 0.643 0.529 0.571 0.471 0.943 0.157 0.500 1.000
Aco-4 1 0.914 1.000 0.957
2 0.857 0.271 0.382 1.000 1.000 0.200 0.143 0.186 0.471 0.086 0.271 0.043
3 0.143 0.729 0.618 0.800 0.857 0.814 0.529 1.000 1.000 0.914 0.729 0.086
LAP Lap-1 1 0.257 0.286 0.143
2 0.500 0.500 0.132 0.471 1.000 0.200 0.429 0.871 0.228 0.014 0.757 0.129 0.071 0.957 0.829
3 0.242 0.243 0.868 0.529 0.571 0.129 0.386 0.457 0.957 0.200 0.757 0.929
4 0.258 0.800 0.614 0.029 0.029 0.043 0.114 0.000 0.043
5 *0.029
6PGD 6Pgd-1 1 1.000 1.000 1.000 1.000 1.000 1.000 0.400 1.000 1.000 1.000 1.000 1.000 1.000 0.429 0.700 1.000
2 0.600 0.571 0.300
6Pgd-2 1 0.814 1.000
2 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 0.186 1.000