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But for all the species, the diversity within popula- villosa showed a greater diversity within populations
tions (Hs) was greater than the diversity among for Lap-1 and B. incana for 6Pgd-1. The loci in-
populations (Dst) in the same proportion (Figure 3). volved in the populations differentiation (Gst) were
Although, the differentiation in Hs and Dst was also different: Pgm-2 and Aco-4 for B. villosa and
similar according to the species, the structure of the Lap-1 and Pgm-1 for B. incana.
genetic diversity at the studied loci was different. For
B. rupestris (Table 5a), the total diversity (Ht) and the
diversity within populations (Hs) was greater at the Genetic distances
Aco-3 and Lap-1 loci. For this species, Pgm-2 and
6Pgd-1 were important for the differentiation among Matrix of genetic distance (Table 6), shows that
the populations (Gst). The total diversity (Ht) was distance values among B. villosa populations (pop 1
greater at Lap-1 and Pgm-2 loci for both B. villosa to 6) were comprised between 0.06 and 0.26 and
(Table 5b) and B. incana (Table 5c) species. But B. ranged from 0.05 to 0.20 among B. rupestris popula-

Table 5. Diversity index: total genetic diversity (Ht), genetic diversity within populations (Hs), genetic dufferentiation (Gst) for each species.
a) B. rupestris (7 population)
System Locus Ht Hs Dst Gst
PGI Pgi-2 0.032 0.029 0.003 0.099
PGM Pgm-3 0.020 0.019 0.001 0.049
Pgm-1 0.254 0.152 0.102 0.401
Pgm-2 0.376 0.102 0.274 0.729
ACO Aco-1 0.130 0.097 0.033 0.254
Aco-2 0.397 0.274 0.123 0.311
Aco-3 0.610 0.432 0.178 0.292
Aco-4 0.276 0.228 0.047 0.172
LAP Lap-1 0.620 0.387 0.233 0.376
6PGD 6Pgd-1 0.157 0.068 0.088 0.562
Mean 0.287 0.178 0.108 0.324
b) Brassica villosa (6 populations)
System Locus Ht Hs Dst Gst
PGI Pgi-2 0.083 0.0641 0.019 0.226
PGM Pgm-1 0.332 0.265 0.067 0.201
Pgm-2 0.499 0.238 0.261 0.522
ACO Aco-1 0.393 0.219 0.174 0.442
Aco-2 0.455 0.239 0.217 0.476
Aco-3 0.248 0.188 0.060 0.243
Aco-4 0.472 0.238 0.233 0.494
LAP Lap-1 0.650 0.383 0.267 0.411
Mean 0.391 0.229 0.162 0.377
c) Brassica incana (2 populations)
System Locus Ht Hs Dst Gst
PGI Pgi-2 0.311 0.237 0.074 0.239
PGM Pgm-1 0.485 0.142 0.343 0.706
Pgm-2 0.582 0.356 0.226 0.389
ACO Aco-2 0.029 0.028 0.001 0.015
Aco-3 0.375 0.250 0.125 0.333
Aco-4 0.082 0.078 0.004 0.055
LAP Lap-1 0.519 0.107 0.412 0.793
6PGD 6Pgd-1 0.492 0.455 0.037 0.075
6Pgd-2 0.169 0.151 0.017 0.102
Mean 0.338 0.200 0.138 0.300