Page 3 - Korn_alii_2006

M. Korn et al. • Sister species within Triops cancriformis

Table 1 Geographical origin and museum
Taxon Voucher number Geographical origin Haplotype group
specimen tissue voucher numbers (MTD-
TW) of specimens of which we obtained 16S T. c. cancriformis 8, 9 Italy, Sicily, Gela C. Eur
sequences in this study and short names of T. c. cancriformis 34 Italy, Favignana Island, pond 1 C. Eur
haplotype groups. (For definition of T. c. cancriformis 256 Italy, Favignana Island, pond 2 C. Eur
haplotype groups see Appendix 1). T. c. cancriformis 101, 102 Tunisia, Bou Salem C. Eur
T. c. cancriformis 47, 48, 49 Germany, Ingolstadt C. Eur
T. c. cancriformis 109, 110 Malta C. Eur
T. c. cancriformis 249, 250 Serbia, Melenci, pond 1 C. Eur
T. c. cancriformis 251, 252, 253 Serbia, Melenci, pond 2 C. Eur
T. c. cancriformis 22, 23, 24 Italy, Ustica Island Sicily
T. c. cancriformis 25, 26, 27 Italy, Sicily, Custonaci Sicily
T. c. cancriformis 28, 29, 30 Tunisia, Jendouba Sicily
T. c. cancriformis 74 Hungary, Kunszentmiklós Hungary
T. c. cancriformis 254 Hungary, Poroszló Hungary
T. c. cancriformis 255 Hungary, Tiszabercel Hungary
T. c. cancriformis 50, 51, 52 Austria, commercial kit Austria
T. c. cancriformis 156 United Arab Emirates, Sharjah Austria
T. c. cancriformis 246, 247, 248 Russia, Uljanowsk Russia
T. c. simplex 10, 11, 12 Tunisia, Tunis Tunisia
T. c. simplex 31, 32, 64 Tunisia, Jendouba Tunisia
T. c. simplex 15 Tunisia, Kairouan, pond 064 Interm.
T. c. simplex 146, 147 Morocco, Ain-Benimathar T.c.s. M.
T. c. simplex 20, 21, 103, 104, 105, 106 Spain, Girona N. Spain
T. c. mauritanicus 1, 2, 3 Morocco, El-Hajeb, pond 058 M 058
T. c. mauritanicus 4, 37 Morocco, Timahdite Rabat
T. c. mauritanicus 5, 6 Morocco, Rabat, pond 059 Rabat
T. c. mauritanicus 39 Morocco, Rabat, pond 060 Rabat
T. c. mauritanicus 126 Morocco, Casablanca, pond 002 Casab.
T. c. mauritanicus 136, 137 Morocco, Casablanca, pond 005 Casab.
T. c. mauritanicus 134, 135 Morocco, Safi S. W. M.
T. c. mauritanicus 138, 139 Morocco, Essaouira, pond 049 S. W. M.
T. c. mauritanicus 141 Morocco, Essaouira, pond 050 S. W. M.
T. c. mauritanicus 142, 143 Morocco, High Atlas S. of Marrakech H. Atlas
T. c. mauritanicus 144, 145 Morocco, Mrirt, pond 056 M 056
T. c. mauritanicus 54, 55 Spain, Extremadura, pond Gitanilla Gitanilla
T. c. mauritanicus 158–169 Spain, Extremadura, from 4 pools S. Spain
T. c. mauritanicus 65, 66, 67 Spain, Sevilla S. Spain
T. c. mauritanicus 68, 69, 70 Spain, Huelva S. Spain
T. c. mauritanicus 18, 19, 76, 77, 78 Portugal, Sagres Portugal
T. c. ssp. ‘intermediate’ 13, 14 Tunisia, Kairouan, pond 063 Interm.

Determination of specimens which all determined specimens showed a smooth carina,
The characters given by Longhurst (1955) were used to were nevertheless classified as T. c. cancriformis because of the
determine specimens of T. cancriformis provisionally to absence of males in these samples and the ability of indi-
subspecies (see Table 3). viduals to reproduce in the absence of males, which we tested
The northern Spanish population investigated here had in all of the populations in question. Unisexual Tunisian
already been classified as T. c. simplex by Margalef (1951, populations (one with specimens showing carinal spines, one
1953) and Alonso (1985, 1996); thus we only determined a in which almost all specimens showed a smooth carina) were
few specimens. These showed the typical morphological also classified as T. c. cancriformis. Their ability to reproduce
features of this subspecies (but see Results section below). in the absence of males was tested using the population in
Longhurst (1955) had reported the absence of T. c. simplex which most specimens lacked carinal spines. One population
from the Iberian Peninsula, but did not investigate specimens from Tunisia (pond 063, Kairouan; Table 1) could not be
from the same population (his classification was based on a classified using the characters given in the literature, because
population from Valencia in eastern Spain). specimens showed a mixture of characters of all subspecies.
All specimens with large furcal spines were classified as This population had an equal distribution of sexes (53%
T. c. mauritanicus. Central European and Italian samples, of males, n = 17).

© 2006 The Authors. Journal compilation © 2006 The Norwegian Academy of Science and Letters • Zoologica Scripta, 35, 4, July 2006, pp301–322 303