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632 A. Scialabba et al.
acid and show very limited variation in FA composi- Acknowledgements
tion (Röbbelen & Thies 1980).
The authors thank the Germplasm Bank of the
FA composition was reported for B. incana,
Botanical Garden of the University of Palermo for
B. rupestris and B. villosa by Velasco et al. (1998)
providing the accessions of Brassicaceae. Special
with the amount of C 22:1 exceeding 50% of T-
thanks go to Prof. F. M. Raimondo for the critical
FA, while in the species analysed in the present
review of the manuscript and to Dr Massimo
study the results never exceeded 25%; however, the
Migliorini for helpful suggestions in the statistical
absolute value of FA cannot be compared since the
analysis. Financial support was provided by Palermo
authors did not report such data. The correlation
University (Fondi di Ateneo), by the Italian MIUR
between T-FA content and C 18:1, C 18:2 and C
(PRIN 2007) and by Siena University (P.A.R.).
22:1 FA suggests that in the seeds of the taxa analy-
sed here, unsaturated FA content was prevalent.
The positive correlation between T-Toc and γ-Toc References
and the negative correlation between T-Toc and α-
Appelqvist LA, Kornfeldt AK, Wennerholm JE. 1981. Sterols
Toc indicate that in the seeds analysed T-Toc and steryl esters in some Brassica and Sinapis seeds.
mainly accounts for γ-Toc synthesis. Since γ-Toc is Phytochemistry 20: 207–210.
the immediate precursor of α-Toc, the availability Azuma K, Ippoushi K, Ito H, Higasho H, Terao J. 1999. Evalu-
of the last enzyme needed for α-Toc synthesis, γ- ation of the antioxidants activity of vegetable extracts in
linoleic emulsion and phospholipids bilayers. J Sci Food Agric
methyltransferase could be rate-limiting (Della
79: 2010–2016.
Penna & Pogson 2006). Similarly, the positive Baecher CWW. 1994. Cancer preventive properties of Brassica
Downloaded By: [Scialabba, A.] At: 20:01 10 October 2010
correlation between the very long-chain C 22:1 and oleracea. Rev Am J Clin Nutr 59: 1166–1170.
C 18:1 FA clearly confirms that the former is Bellani LM, Salvini L, Scialabba A. 2009. Characterization of
formed by elongation of the latter. A positive corre- Brassica fruticulosa Cyr. seeds. Bocconea 23: 451–457.
Conti F, Abbate G, Alessandrini A, Blasi C. 2005. An annotated
lation between C 18:3, T-Toc and γ-Toc and a
check-list of the Italian vascular flora. Roma: Palombi. p. 420.
negative correlation between α- and γ-Toc were Della Penna D, Pogson BJ. 2006. Vitamin synthesis in plants: Toco-
observed in 91 species of Brassicaceae (Goffman et pherols and carotenoids. Annu Rev Plant Biol 57: 711–738.
al. 1999) and in vegetable oils (Kamal-Eldin & Drissi A, Girona J, Cherki M, Godàs G, Derouiche A, El Messal
Andersson 1997). M, et al. 2004. Evidence of hypolipemiant and antioxidant
properties of argan oil derived from the argan tree (Argania
The presence of brassicasterol is typical for the
spinosa). Clin Nutr 23: 1159–1166.
Brassicaceae family (Gül & Amar 2006) and the Evangelou A, Kalpouzos G, Karkabounas S, Liasko R, Nonni A,
relative percentages of phytosterol were in line with Stefanou D, et al. 1997. Dose-related preventive and
those reported for B. napus L., B. campestris L. and therapeutic affects of antioxidants anticarcinogens on
B. juncea (L.) Coss. (Appelqvist et al. 1981). experimentally induced malignant tumors in Wistar rats.
Cancer Lett 115: 105–111.
However, FA and phytosterol profiles were not
Faulkner K, Mithen R, Williamson G. 1998. Selective increase
found to be useful to discriminate species as no of the potential anticarcinogen 4-methylsulphinylbutyl
correlation was detected. The chemical composition glucosinolate in broccoli. Carcinogenesis 19: 605–609.
of the seeds analysed is interesting for the presence Geraci A, Divaret I, Raimondo FM, Chèvre AM. 2001. Genetic
of Toc, phytosterols and unsaturated FA, all relationships between Sicilian wild populations of Brassica
analysed with RAPD markers. Plant Breed 120: 193–196.
compounds with antioxidant effects and positively
Giardina G, Raimondo FM, Spadaro V. 2007. A catalogue of
correlated with a reduced risk of cardiovascular plants growing in Sicily. Bocconea 20: 15–582.
diseases, a reduced level of cholesterol (Drissi et al. Goffman FD, Becker HC. 2002. Genetic variation of tocopherol
2004; Gül & Amar 2006) and ameliorated diabetic content in a germplasm collection of Brassica napus L.
nephropathy (Kataya & Hamza 2008). The Toc Euphytica 125: 189–196.
Goffman FD, Thies W, Velasco L. 1999. Chemotaxonomic value
content investigated in a collection of B. napus
of tocopherols in Brassicaceae. Phytochemistry 50: 793–798.
(Goffman & Becker 2002) and B. fruticulosa Cyr. Grob R, Barry E. 2004. Modern practice of gas chromatography.
seeds (Bellani et al. 2009) was lower than in the B. Hoboken, NJ: Wiley. p. 1064.
villosa group, except for B. villosa subsp. brevisiliqua. Gül MK, Amar S. 2006. Sterols and the phytosterol content in
Hybrids between commercial broccoli and B. villosa oilseed rape (Brassica napus L.). J Cell Mol Biol 5: 71–79.
Harbert DJ. 1972. A contribution to the cytotaxonomy of
subsp. villosa and B. v. subsp. drepanensis are fully
Brassica (Cruciferae) and its allies. Bot J Linn Soc 65: 1–23.
fertile and have developed lines with enhanced levels Heywood VH, Zohary D. 1995. A catalogue of the wild rela-
of glucosinolates (Faulkner et al. 1998). B. villosa tives of cultivated plants native to Europe. Fl Medit 5:
subsp. villosa, B. v. subsp. bivoniana, B. v. subsp. 375–415.
drepanensis and B. v. subsp. tinei had the highest T- Jones PJ, Raeini-Sarjaz M, Ntanios FY, Vanstone CA, Feng JY,
Parsons WE. 2000. Modulation of plasma lipid levels and
Toc, γ-Toc and phytosterol contents, suggesting that
cholesterol kinetics by phytosterol versus phytostanol esters.
they could be exploited in breeding programmes to J Lipid Res 41: 697–705.
develop genotypes with enhanced antioxidant capac- Kamal-Eldin AQ, Andersson R. 1997. A multivariate study of
ities and nutritional value. the correlation between tocopherol content and fatty acid