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954 G. CARUSO AND R. ZACCONE
(a) –1 –1 (b) –1 –1 (c) –1 –1
µg C l h µg C l h µg C l h
0 10 0 1 2 0 1 2
0 0 0
5
5 5
10
Depth (m) 10 10 15
20
15 15
25
20 20 30
Fig. 3 The Adriatic Sea (Prisma 1 project): vertical pro®les of LAP activity at two coastal sites, Station S1 (a) and Station S2 (b), affected
by Po and Adige river runoff, and at one off-shore station, S3 (c), sampled in April (W), July (&) and October (~), respectively. Error
bars indicate S.D. from the mean value (n 3)
from coastal to off-shore stations, the spatial distribution of ferences among areas (F 541, P < 005, n 116), seasons
LAP was characterized in both areas by a decreasing trend, (F 348, P < 005, n 60) and years (F 939, P < 005,
in agreement with the trophic gradient but inverse to the n 60).
salinity gradient. As far as the relationship between LAP and some biotic
Enzyme activity varied on a seasonal scale, with average and abiotic parameters is concerned (Table 2), Pearson cor-
values 15 times higher in summer than in winter. The relation coef®cients showed that LAP levels were some-
ANOVA test, however, did not reveal any signi®cant dif- times signi®cantly related to viable heterotrophic bacterial
Table 1 The Adriatic Sea (Prisma 1 project). Coef®cients of correlation between LAP and the main physico-chemical and
microbiological data recorded in surface and along the water column at two coastal sites (S1 and S2) and at one off-shore station (S3),
respectively. In parenthesis the number of pairs of data is reported
Station S1 Station S2 Station S3
All surface data
Water Water Water
0 m (3) column (14) 0 m (3) column (14) 0 m (3) column (15) (9)
Temperature 083 045 082 037 ÿ 093 009 040
Salinity 043 035 ÿ 089 028 023 051 ÿ009
Viable heterotrophic bacteria * 0996 ** 089 004 010 * 0990 037 ** 093
Bacterial carbon production ** 0999 ** 097 ** 0999 038 027 022 ** 097
Chlorophyll a 078 ** 090 043 022 * 0993 010 * 079
POC *098 ÿ008 013
*Signi®cant value at P < 005; **Signi®cant value at P < 001.
= 2000 The Society for Applied Microbiology, Journal of Applied Microbiology, 89, 951ÿ959