Page 6 - Geraci_al_2004

142 villosa showed a greater diversity within populations
for Lap-1 and B. incana for 6Pgd-1. The loci in-
But for all the species, the diversity within popula- volved in the populations differentiation (Gst) were
tions (Hs) was greater than the diversity among also different: Pgm-2 and Aco-4 for B. villosa and
populations (Dst) in the same proportion (Figure 3). Lap-1 and Pgm-1 for B. incana.

Although, the differentiation in Hs and Dst was Genetic distances
similar according to the species, the structure of the
genetic diversity at the studied loci was different. For Matrix of genetic distance (Table 6), shows that
B. rupestris (Table 5a), the total diversity (Ht) and the distance values among B. villosa populations (pop 1
diversity within populations (Hs) was greater at the to 6) were comprised between 0.06 and 0.26 and
Aco-3 and Lap-1 loci. For this species, Pgm-2 and ranged from 0.05 to 0.20 among B. rupestris popula-
6Pgd-1 were important for the differentiation among
the populations (Gst). The total diversity (Ht) was
greater at Lap-1 and Pgm-2 loci for both B. villosa
(Table 5b) and B. incana (Table 5c) species. But B.

Table 5. Diversity index: total genetic diversity (Ht), genetic diversity within populations (Hs), genetic dufferentiation (Gst) for each species.

a) B. rupestris (7 population)

System Locus Ht Hs Dst Gst

PGI Pgi-2 0.032 0.029 0.003 0.099
PGM Pgm-3 0.020 0.019 0.001 0.049
Pgm-1 0.254 0.152 0.102 0.401
ACO Pgm-2 0.376 0.102 0.274 0.729
Aco-1 0.130 0.097 0.033 0.254
LAP Aco-2 0.397 0.274 0.123 0.311
6PGD Aco-3 0.610 0.432 0.178 0.292
Mean Aco-4 0.276 0.228 0.047 0.172
Lap-1 0.620 0.387 0.233 0.376
6Pgd-1 0.157 0.068 0.088 0.562
0.287 0.178 0.108 0.324

b) Brassica villosa (6 populations)

System Locus Ht Hs Dst Gst

PGI Pgi-2 0.083 0.0641 0.019 0.226
PGM Pgm-1 0.332 0.265 0.067 0.201
ACO Pgm-2 0.499 0.238 0.261 0.522
Aco-1 0.393 0.219 0.174 0.442
LAP Aco-2 0.455 0.239 0.217 0.476
Mean Aco-3 0.248 0.188 0.060 0.243
Aco-4 0.472 0.238 0.233 0.494
Lap-1 0.650 0.383 0.267 0.411
0.391 0.229 0.162 0.377

c) Brassica incana (2 populations)

System Locus Ht Hs Dst Gst

PGI Pgi-2 0.311 0.237 0.074 0.239
PGM Pgm-1 0.485 0.142 0.343 0.706
ACO Pgm-2 0.582 0.356 0.226 0.389
Aco-2 0.029 0.028 0.001 0.015
LAP Aco-3 0.375 0.250 0.125 0.333
6PGD Aco-4 0.082 0.078 0.004 0.055
Mean Lap-1 0.519 0.107 0.412 0.793
6Pgd-1 0.492 0.455 0.037 0.075
6Pgd-2 0.169 0.151 0.017 0.102
0.338 0.200 0.138 0.300