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Monitoring invasive algae
            For each different habitat, the coverage of invasive algae
            may be quantified by using 25 cm x 25 cm quadrats, each
            subdivided into 25 subquadrats of 5 cm x 5 cm (Cebrian
            et al., 2000; Fig. 13). In each habitat type, divers position
                                                 2
            20 quadrats (covering a total area of 1.25 m ) randomly
            over the substrate and record the number of subquadrats
            in which the specific invasive alga occurs.
            Monitoring sessile invertebrates and
            species with scattered distribution

            Bathymetric transects performed in different habitats at
            each sampling station may firstly identify the depth at
            which other invasive species are most abundant. At each
            depth, two transects (50 m x 1 m) located randomly
            should be monitored by a scuba-diving team. In those
            cases where invasive species may be of a considerable
            size (e.g. the invasive coral  Oculina patagonica), only
            colonies or individuals with at least 50% of their surface
            area lying within the belt transect should be considered
            and counted to avoid bias in the sampling (Nugues and
            Roberts, 2003).
            Monitoring invasive fishes

            At each sampling station, the abundance and size of any
            invasive fish should be recorded along transects. An
            observer should dive at an approximately constant speed
            along three 25 m x 5 m transects at each sampling station  Fig. 16. Monitoring can be used to illustrate the extent and the
            and at a fixed depth (where invasive fishes are most  depth range of invasive colonizers over time. Some species, such
                                                                  as Caulerpa racemosa var. cylindracea, might start their
            abundant).
                                                               colonization at greater depths and move to shallower waters after
                                                               a few years, making it more difficult to detect them if surveys are
            Along each transect the observer will identify the species,  only conducted in shallow environments.
            count the number of individuals observed and estimate the
            approximate size of all individuals (in 2 cm increments of
            total length, TL). Fish biomass (g wet weight m-2) can be
            estimated from size data using length-weight relationships
            from the available literature and databases (Froese and
            Pauly, 2009).



























             Fig. 15. Semi-quantitative method to calculate percentage of  Stephanolepis diaspros. Photo: A. Can – www.alpcan.com
                  cover of benthic species. Photo: E. Ballesteros




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